HLB

Reversed Phase Polymeric

MCX

Mixed Mode Strong Cation Exchanger, Polymeric

MAX

Mixed Mode Strong Anion Exchanger, Polymeric

WAX

Mixed Mode Weak Anion Exchanger, Polymeric

MCX

Mixed Mode Weak Anion Exchanger, Polymeric

ALUMINA BASIC

Mixed Mode Weak Anion Exchanger, Polymeric

The new generation Affinity SPE sorbents are spherical with a uniform particle size. They also have a high surface area and high density of functional groups on the particle surface.

Broad application pH range, optimized pore size, and uniform surface functional groups. These sorbents therefore readily satisfy the need for high sensitivity in analyzing different drugs and chemicals.

Affinity SPE’s unique particle technology not only ensures the consistency of the sorbents size and internal pore structure, but also brings about high recovery and good reproducibility for specific adsorption...

General Methodology

A generic Solid Phase Extraction (SPE) protocol is recommended when working with a diverse range of acidic, basic, and neutral compounds to ensure the effective removal of unretained matrix components like salts, lipids, and proteins. This process typically involves conditioning the SPE cartridge, loading the sample to allow the matrix to pass through while retaining the target compounds, washing to eliminate impurities, and finally eluting the purified acidic, basic, and neutral compounds. The goal is to achieve consistent and reproducible extraction by isolating the compounds of interest from complex sample matrices, ensuring accurate and reliable analytical results.

For basic compounds, the Affinity mixed-mode MCX generic method is recommended, as it efficiently removes unretained matrix components in reverse-phase mode, followed by the elimination of retained non-basic interferences in ion-exchange mode. Similarly, for acidic compounds, the Affinity MAX generic method employs the same mixed-mode approach, ensuring that matrix interferences are thoroughly removed while the target compounds are selectively retained and purified. This dual-phase method enhances the reliability and reproducibility of the extraction process, making it ideal for handling complex samples with both basic and acidic compounds.