Generic SPE Protocol
A generic Solid Phase Extraction (SPE) protocol is recommended when working with a diverse range of acidic, basic, and neutral compounds to ensure the effective removal of unretained matrix components like salts, lipids, and proteins. This process typically involves conditioning the SPE cartridge, loading the sample to allow the matrix to pass through while retaining the target compounds, washing to eliminate impurities, and finally eluting the purified acidic, basic, and neutral compounds. The goal is to achieve consistent and reproducible extraction by isolating the compounds of interest from complex sample matrices, ensuring accurate and reliable analytical results.
Affinity™ Mixed-Mode
For basic compounds, the Affinity mixed-mode MCX generic method is recommended, as it efficiently removes unretained matrix components in reverse-phase mode, followed by the elimination of retained non-basic interferences in ion-exchange mode. Similarly, for acidic compounds, the Affinity MAX generic method employs the same mixed-mode approach, ensuring that matrix interferences are thoroughly removed while the target compounds are selectively retained and purified. This dual-phase method enhances the reliability and reproducibility of the extraction process, making it ideal for handling complex samples with both basic and acidic compounds.
