Bio-Analytical Applications

Explore our growing library of validated applications using Affinity™ SPE technology.

Published Methods

(Click Application Name for detailed method information where available)

Application	Analyte(s)	LLOQ	Unit	Uniqueness	SPE Method	LCMS/MS
Semaglutide	Semaglutide	0.5	ng/ml	Lowest LLOQ	Affinity SPE, MAX Pro	API-5500
Tirzepatide	Tirzepatide	0.25	ng/ml	Lowest LLOQ	Affinity SPE, MAX Pro	API-5500
Avatrombopag	Avatrombopag	1.0	ng/ml	-	Affinity SPE, MAX Pro	API-4000
Fidaxomycin	Fidaxomycin OP1118	50 200	pg/ml	Single Method	Affinity SPE, HLB	API-5500
Vitamin D3	1, 25 dihydroxy 25-hydroxy	5 5	pg/ml ng/ml	Single Method	Affinity SPE, HLB	API-4000
Empagliflozin + Sitagliptin	Empagliflozin Sitagliptin	1 2	ng/ml	Single Method	Affinity SPE, HLB	API-4000
Sitagliptin + Metformin	Sitagliptin Metformin	2 10.0	ng/ml	Single Method	Affinity SPE, HLB	API-4000
Mesalamine	N-Propionyl 5-Amino Salicylic Acid	0.5	ng/ml	-	Affinity SPE, MAX	API-4000
Tacrolimus	Tacrolimus	100	pg/ml	-	Affinity SPE, HLB	API-5500
Lutein + Zeaxanthin	Lutein Zeaxanthin	10 10	ng/ml	Single Method	Affinity SPE, C18/HLB	API-5500
Epinephrine	Epinephrine	5	pg/ml	-	Affinity SPE, WCX/HLB	API-5500/API-6500
Empagliflozin + Pioglitazone	Empagliflozin Pioglitazone	- -	ng/ml	Single Method	Affinity SPE, MCX	API-4000
Amoxicillin + Clavulanic acid	Amoxicillin Clavulanic acid	10 5	ng/ml	Single Method	Affinity SPE, HLB	API-4000
Montelukast	Montelukast	-	-	-	Affinity SPE, HLB	API-4000
Dydrogesterone	Dydrogesterone	50	pg/ml	-	Affinity SPE, C18	API-4000
Levothyroxine	Levothyroxine	20	ng/ml	Serum estimation	Affinity SPE, HLB	API-4000
Paclitaxel	Total Paclitaxel Unbound Paclitaxel	- -	-	-	Affinity SPE, HLB	API-4000
Everolimus	Everolimus in whole blood	0.5	ng/ml	-	Affinity SPE, HLB	API-5500
Dabigatran	Free Dabigatran Free + Dabigatran (Total)	1.0	pg/ml	-	Affinity SPE, HLB	API-4000
Linagliptin	Linagliptin	50.0	pg/ml	-	Affinity SPE, MCX	API-4000
Mirabegran	Mirabegran	0.1	ng/ml	-	Affinity SPE, HLB	API-5500
Rupatadine	Rupatadine	50.0	pg/ml	-	Affinity SPE, MCX	API-4000
Selexipag	Selexipag ACT-333679 (Metabolite)	0.1	ng/ml	Single Run	Affinity SPE, HLB	API-5500
Saxagliptin	Saxagliptin 5-Hydroxy Saxagliptin	0.1 0.2	ng/ml	Single Run	Affinity SPE, MCX	API-4000
Tenofovir	Tenofovir Alfenamide Tenofovir	1.0 0.3	ng/ml	Single Run	Affinity SPE, MCX	API-4000
Tacrolimus in whole blood	Tacrolimus in whole blood	0.1	ng/ml	-	Affinity SPE, HLB	API-5500
Vortioxetine	Vortioxetine	0.1	ng/ml	-	Affinity SPE, HLB	API-4000

General Affinity™ SPE Information & Protocols

The following general information and starting protocols apply to most Affinity SPE products.

Affinity, next-generation Solid Phase Extraction (SPE)

GS Labloure ”Affinity” products revolutionize Solid Phase Extraction (SPE). Affinity SPE product range provides greater reproducibility with cleaner, more consistent extracts. Affinity SPE products provide unparalleled performance characteristics compared to liquid-liquid extraction, phospholipid removal and protein precipitation products.

This includes:

higher levels of reproducibility
higher levels of extract cleanliness
reduced solvent requirements
increased sensitivity
greater sample throughput

The proprietary manufacturing process involved in the production of Affinity cartridges and plates, provides an SPE product which eliminates issues normally associated with conventional loose-packed SPE.

AFFINITY product methods

The generic AFFINITY product methods outlined below are designed to be a starting point for most sample extraction protocols. Generic method protocol for GS Labloure’s Affinity SPE cartridges and 96 well plate formats:

Affinity HLB cartridge (Reversed-phase): Condition: 500 μL methanol; Equilibrate: 500 μL water; Load: 100 to 500 μL of sample at 1 ml per minute; Wash 1: 500 μL water; Wash 2: 500 μL 5% methanol in water; Elute: 200 μL - 500 μL methanol

Affinity MCX cartridge (Mixed-mode strong cation exchanger): Condition: 500 μL methanol; Equilibrate: 500 μL water with 1% formic acid; Load: 100 to 500 μL of sample at 1 ml per minute containing 1% formic acid; Wash 1: 500 μL water with 1% formic acid; Wash 2: 500 μL methanol with 1% formic acid; Elute: 200 μL - 500 μL methanol with 2% ammonium hydroxide

Affinity MAX cartridge (Mixed-mode strong anion exchanger): Condition: 500 μL methanol; Equilibrate: 500 μL water with 1% ammonium hydroxide; Load: 100 to 500 μL of sample at 1 ml per minute containing 1% ammonium hydroxide; Wash 1: 500 μL water with 1% ammonium hydroxide; Wash 2: 500 μL methanol with 2% ammonium hydroxide; Elute: 200 μL - 500 μL methanol with 1% formic acid

Affinity WAX cartridge (Mixed-mode weak anion exchanger): Condition: 500 μL methanol; Equilibrate: 500 μL water with 1% formic acid; Load: 100 to 500 μL of sample at 1 ml per minute containing 1% formic acid; Wash 1: 500 μL water with 1% formic acid; Wash 2: 500 μL methanol with 1% formic acid; Elute: 200 μL - 500 μL methanol with 2% ammonium hydroxide

Affinity WCX cartridge (Mixed-mode weak cation exchanger): Condition: 500 μL methanol; Equilibrate: 500 μL water with 1% ammonium hydroxide; Load: 100 to 500 μL of sample at 1 ml per minute containing 1% ammonium hydroxide; Wash 1: 500 μL water with 1% ammonium hydroxide; Wash 2: 500 μL methanol with 1% ammonium hydroxide; Elute: 200 μL - 500 μL methanol with 1% formic acid

Summary

Compared to conventional SPE loose-packed products...

Product Information

Affinity products are available in 25 mg/mL cartridges and 25/10 mg/mL 96 well plate formats.

Affinity SPE Cartridges

Description	Bed weight	Column volume (mL)	Cat. no.	Quantity
Affinity HLB	25 mg	1 mL	-001	100 pk
Affinity MCX	25 mg	1 mL	-002	100 pk
Affinity MAX	25 mg	1 mL	-003	100 pk
Affinity WCX	25 mg	1 mL	-004	100 pk
Affinity WAX	25 mg	1 mL	-005	100 pk
Affinity HLB-Pro	25 mg	1 mL	-001	100 pk
Affinity MCX-Pro	25 mg	1 mL	-002	100 pk
Affinity MAX-Pro	25 mg	1 mL	-003	100 pk
Affinity WCX-Pro	25 mg	1 mL	-004	100 pk
Affinity WAX-Pro	25 mg	1 mL	-005	100 pk

Affinity 96 Well Plates

Description	Bed weight	Column volume (mL)	Cat. no.	Quantity
Affinity HLB	25 or 10 mg	1 mL	-001	1
Affinity MCX	25 mg	1 mL	-002	1
Affinity MAX	25 mg	1 mL	-003	1
Affinity WCX	25 mg	1 mL	-004	1
Affinity WAX	25 mg	1 mL	-005	1

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